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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBX2 Monoclonal antibody

CBX2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3H3

Cat no : 68244-1-Ig

Synonyms

SRXY5, M33, CDCA6, 1D3H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBX2 Monoclonal antibody (68244-1-Ig)

    WB analysis using 68244-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68244-1-Ig (CBX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CBX2 Monoclonal antibody (68244-1-Ig)

    IF Staining of HEK-293 using 68244-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CBX2 antibody (68244-1-Ig, Clone: 1D3H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68244-1-Ig targets CBX2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CBX2 fusion protein Ag8311 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 2 (Pc class homolog, Drosophila)
Calculated molecular weight 56 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC004252
Gene symbol CBX2
Gene ID (NCBI) 84733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The chromobox homolog protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.

Protocols

Product Specific Protocols
WB protocol for CBX2 antibody 68244-1-IgDownload protocol
IF protocol for CBX2 antibody 68244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols