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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CBX2 Monoclonal antibody

CBX2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D3H3

Cat no : CL488-68244

Synonyms

SRXY5, M33, CDCA6, 1D3H3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated CBX2 Monoclonal anti (CL488-68244)

    FC experiment of HEK-293 using CL488-68244

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CBX2 (CL488-68244, Clone:1D3H3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68244 targets CBX2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CBX2 fusion protein Ag8311 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 2 (Pc class homolog, Drosophila)
Calculated molecular weight 56 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC004252
Gene symbol CBX2
Gene ID (NCBI) 84733
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The chromobox homolog protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CBX2 antibody CL488-68244Download protocol
Standard Protocols
Click here to view our Standard Protocols