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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of NIH/3T3 using 66446-1-Ig (same clone as 66446-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of HEK-293 using 66446-1-Ig (same clone as 66446-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of Jurkat using 66446-1-Ig (same clone as 66446-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of RAW 264.7 using 66446-1-Ig (same clone as 66446-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of HeLa using 66446-1-Ig (same clone as 66446-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    IF Staining of HepG2 using 66446-1-Ig (same clone as 66446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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CBX3 Monoclonal antibody, PBS Only

CBX3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66446-1-PBS
Clone No.1A5A3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66446-1-PBS targets CBX3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CBX3 fusion protein Ag19112 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC000954
Gene Symbol CBX3
Gene ID (NCBI) 11335
RRIDAB_2881815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13185
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins