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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBX3 Monoclonal antibody

CBX3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A3

Cat no : 66446-1-Ig

Synonyms

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of NIH/3T3 using 66446-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of RAW 264.7 using 66446-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of HEK-293 using 66446-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of Jurkat using 66446-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CBX3 Monoclonal antibody (66446-1-Ig)

    WB analysis of HeLa using 66446-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBX3 Monoclonal antibody (66446-1-Ig)

    IF Staining of HepG2 using 66446-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66446-1-Ig targets CBX3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CBX3 fusion protein Ag19112 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC000954
Gene symbol CBX3
Gene ID (NCBI) 11335
RRIDAB_2881815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Protocols

Product Specific Protocols
WB protocol for CBX3 antibody 66446-1-IgDownload protocol
IF protocol for CBX3 antibody 66446-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

Authors - Dan Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

ILIAS (Verified Customer) (05-26-2023)

The 66446-1-Ig worked very well in both Western Blot and Immunofluorescence microscopy experiments

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 (WB), 1:500(IF)
  • Cell Tissue Type: HeLa and MCF7 cells
CBX3 Antibody Western Blot,Immunofluorescence validation (1:2000 (WB), 1:500(IF) dilution) in HeLa and MCF7 cells (Cat no:66446-1-Ig)