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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated CBX3 Monoclonal anti (CL488-66446)

    IF Staining of HepG2 using CL488-66446

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CBX3 antibody (CL488-66446, Clone: 1A5A3 ) at dilution of 1:500.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CBX3 Monoclonal anti (CL488-66446)

    FC experiment of HeLa using CL488-66446

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CBX3 (CL488-66446, Clone:1A5A3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated CBX3 Monoclonal antibody

CBX3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66446
Clone No.1A5A3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Modifier 2 protein, HP1Hs gamma, HP1 gamma, HECH, 1A5A3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66446 targets CBX3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CBX3 fusion protein Ag19112 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC000954
Gene Symbol CBX3
Gene ID (NCBI) 11335
RRIDAB_2883338
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13185
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CBX3 antibody CL488-66446Download protocol
FC protocol for CL Plus 488 CBX3 antibody CL488-66446Download protocol
Standard Protocols
Click here to view our Standard Protocols