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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CCAR2 Monoclonal antibody

CCAR2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D10F8

Cat no : CL594-66497

Synonyms

CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CCAR2 Monoclonal antibody (CL594-66497)

    IF Staining of HeLa using CL594-66497

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated CCAR2 antibody (CL594-66497, Clone: 2D10F8 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated CCAR2 Monoclonal antibody (CL594-66497)

    FC experiment of HeLa using CL594-66497

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite®594-conjugated CCAR2 Monoclonal antibody (CL594-66497)

    FC experiment of U2OS using CL594-66497

    1X10^6 U2OS cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells, HeLa cells
Positive FC detected inU2OS cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66497 targets CCAR2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCAR2 fusion protein Ag18374 相同性解析による交差性が予測される生物種
Full Name KIAA1967
Calculated molecular weight 923 aa, 103 kDa
GenBank accession numberBC018269
Gene symbol CCAR2
Gene ID (NCBI) 57805
RRIDAB_2883597
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).

Protocols

Product Specific Protocols
IF protocol for CL594 CCAR2 antibody CL594-66497Download protocol
Standard Protocols
Click here to view our Standard Protocols