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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC18 Polyclonal antibody (30024-1-AP)

    WB analysis using 30024-1-AP (same clone as 30024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30024-1-AP (CCDC18 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using CCDC18 Polyclonal antibody (30024-1-AP)

    IHC staining of rat colon using 30024-1-AP (same clone as 30024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30024-1-AP (CCDC18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using CCDC18 Polyclonal antibody (30024-1-AP)

    IHC staining of rat colon using 30024-1-AP (same clone as 30024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30024-1-AP (CCDC18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30024-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCDC18 Polyclonal antibody (30024-1-AP)

    IF Staining of U2OS using 30024-1-AP (same clone as 30024-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC18 antibody (30024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30024-1-PBS in a different storage buffer formulation.

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CCDC18 Polyclonal antibody, PBS Only

CCDC18 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30024-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30024-1-PBS targets CCDC18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC18 fusion protein Ag32681 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 18
Observed molecular weight151 kDa
GenBank accession numberBC156745
Gene Symbol CCDC18
Gene ID (NCBI) 343099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T9S5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCDC18 (Coiled-coil domain-containing protein 18) is a Protein that in humans is encoded by the CCDC18 gene. CCDC18 has 2 isoforms with the MW of 168 kDa and 151 kDa.