Featured Product
KD/KO Validated

Cyclin B1 Polyclonal antibody

Name: Cyclin B1 Polyclonal antibody
Brand: Proteintech
SKU: 55004-1-AP

Cyclin B1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

262

Review Product

Cat no : 55004-1-AP

Synonyms

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55004-1-AP
Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
WB analysis of HeLa using 55004-1-AP
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.
WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55004-1-AP
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.
WB analysis of HeLa using 55004-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 55004-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 55004-1-AP
IP result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:2000) with HeLa cells lysate 1560 ug.
IHC staining of human skin cancer using 55004-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 55004-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 55004-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of U2OS using 55004-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HeLa using 55004-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Cyclin B1 Polyclonal antibody (55004-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

-/ -

Cat No. 55004-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, U2OS cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 248 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

55004-1-AP targets Cyclin B1 in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	cyclin B1
Calculated molecular weight	48 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	NM_031966
Gene symbol	Cyclin B1
Gene ID (NCBI)	891
RRID	AB_10859790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IHC protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IF protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IP protocol for Cyclin B1 antibody 55004-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration Authors - Jinpeng Du View Article
human	WB	Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis Authors - Wei Zhang View Article
human	WB	Acta Pharm Sin B Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway. Authors - Xiangjin Zheng View Article
mouse	IF	Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. Authors - Weimin Yu View Article
human	WB	Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. Authors - Xiaoyu Ma View Article
human	WB	Autophagy PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma. Authors - Bo Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

WB analysis using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

WB analysis of NIH/3T3 using 55004-1-AP

WB analysis using 55004-1-AP

IP experiment of HeLa using 55004-1-AP

IHC staining of human skin cancer using 55004-1-AP

IHC staining of human skin cancer using 55004-1-AP

IF Staining of HeLa using 55004-1-AP

IF Staining of U2OS using 55004-1-AP

FC experiment of HeLa using 55004-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.

Related Products

CDK1-Specific Polyclonal antibody

Cyclin B2 Polyclonal antibody

Cyclin A2 Polyclonal antibody

PLK1 Polyclonal antibody

CDC25C Polyclonal antibody

Cyclin D1 Monoclonal antibody

P21 Polyclonal antibody

P53 Polyclonal antibody

CDK2 Polyclonal antibody

Cyclin G Polyclonal antibody