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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal (CL488-55004)

    IF Staining of HeLa using CL488-55004

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Cyclin B1 antibody (CL488-55004) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal (CL488-55004)

    FC experiment of HeLa using CL488-55004

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cyclin B1 Polyclonal Antibody (CL488-55004)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-55004

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55004 targets Cyclin B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberNM_031966
Gene Symbol Cyclin B1
Gene ID (NCBI) 891
RRIDAB_3672852
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14635
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cyclin B1 antibody CL488-55004Download protocol
FC protocol for CL Plus 488 Cyclin B1 antibody CL488-55004Download protocol
Standard Protocols
Click here to view our Standard Protocols