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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCR5 Recombinant antibody (82942-1-RR)

    WB analysis using 82942-1-RR (same clone as 82942-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82942-1-RR (CCR5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82942-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82942-1-PBS

    Affinity and Kinetic Characterization of 82942-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82942-1-RR against Human CCR5 were performed. The affinity constant is 0.921 nM.

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CCR5 Recombinant antibody, PBS Only

CCR5 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 82942-1-PBS
Clone No.230266A6

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

C-C CKR-5, CC CKR 5, C-C chemokine receptor type 5, C C CKR 5, C C chemokine receptor type 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82942-1-PBS targets CCR5 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCR5 fusion protein Ag11493 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) receptor 5
Calculated molecular weight 352 aa, 41 kDa
Observed molecular weight45 kDa
GenBank accession numberBC038398
Gene Symbol CCR5
Gene ID (NCBI) 1234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51681
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages and is known to be an important co-receptor for macrophage-tropic viruses, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2, and RANTES.