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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCR5 Recombinant antibody (82942-1-RR)

    WB analysis using 82942-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82942-1-RR (CCR5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82942-1-RR

    Affinity and Kinetic Characterization of 82942-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82942-1-RR against Human CCR5 were performed. The affinity constant is 0.921 nM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CCR5 Recombinant antibody

CCR5 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82942-1-RR
Clone No.230266A6

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

C-C CKR-5, CC CKR 5, C-C chemokine receptor type 5, C C CKR 5, C C chemokine receptor type 5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inU-937 cells, mouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82942-1-RR targets CCR5 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCR5 fusion protein Ag11493 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) receptor 5
Calculated molecular weight 352 aa, 41 kDa
Observed molecular weight45 kDa
GenBank accession numberBC038398
Gene Symbol CCR5
Gene ID (NCBI) 1234
RRIDAB_3670685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages and is known to be an important co-receptor for macrophage-tropic viruses, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2, and RANTES.

Protocols

Product Specific Protocols
WB protocol for CCR5 antibody 82942-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols