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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CCS Monoclonal antibody (68341-1-Ig)

    WB analysis of HepG2 using 68341-1-Ig

    WB result of CCS antibody (68341-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCS transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using CCS Monoclonal antibody (68341-1-Ig)

    WB analysis using 68341-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68341-1-Ig (CCS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CCS Monoclonal antibody (68341-1-Ig)

    IHC staining of mouse brain using 68341-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68341-1-Ig (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCS Monoclonal antibody (68341-1-Ig)

    IF Staining of HepG2 using 68341-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCS antibody (68341-1-Ig, Clone: 3F5E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  • Featured Product
  • KD/KO Validated

CCS Monoclonal antibody

CCS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68341-1-Ig
Clone No.3F5E5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCS

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, A549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68341-1-Ig targets CCS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCS fusion protein Ag18860 相同性解析による交差性が予測される生物種
Full Name copper chaperone for superoxide dismutase
Calculated molecular weight 274 aa, 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC105016
Gene Symbol CCS
Gene ID (NCBI) 9973
RRIDAB_3085065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14618
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

Protocols

Product Specific Protocols
WB protocol for CCS antibody 68341-1-IgDownload protocol
IHC protocol for CCS antibody 68341-1-IgDownload protocol
IF protocol for CCS antibody 68341-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols