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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CCS Monoclonal antibody (68341-1-Ig)

    WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    WB result of CCS antibody (68341-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCS transfected HepG2 cells. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CCS Monoclonal antibody (68341-1-Ig)

    WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68341-1-Ig (CCS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CCS Monoclonal antibody (68341-1-Ig)

    IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68341-1-Ig (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCS Monoclonal antibody (68341-1-Ig)

    IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCS antibody (68341-1-Ig, Clone: 3F5E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

CCS Monoclonal antibody, PBS Only

CCS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68341-1-PBS
Clone No.3F5E5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CCS

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68341-1-PBS targets CCS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCS fusion protein Ag18860 相同性解析による交差性が予測される生物種
Full Name copper chaperone for superoxide dismutase
Calculated molecular weight 274 aa, 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC105016
Gene Symbol CCS
Gene ID (NCBI) 9973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14618
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.