CCT2 Monoclonal antibody
CCT2 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Pig, Rabbit, Rat and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2H6C7
Cat no : 68214-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
68214-1-Ig targets CCT2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rabbit, Rat samples.
| Tested Reactivity | Human, Mouse, Pig, Rabbit, Rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CCT2 fusion protein Ag19264 相同性解析による交差性が予測される生物種 |
| Full Name | chaperonin containing TCP1, subunit 2 (beta) |
| Calculated molecular weight | 535 aa, 57 kDa |
| Observed molecular weight | 50-58 kDa |
| GenBank accession number | BC113516 |
| Gene symbol | CCT2 |
| Gene ID (NCBI) | 10576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCT2 antibody 68214-1-Ig | Download protocol |
| IF protocol for CCT2 antibody 68214-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins |
