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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT2 Monoclonal antibody, PBS Only

CCT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rabbit, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H6C7

Cat no : 68214-1-PBS

Synonyms

99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CCT2 Monoclonal antibody (68214-1-Ig)

    WB analysis using 68214-1-Ig (same clone as 68214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CCT2 Monoclonal antibody (68214-1-Ig)

    IF Staining of NIH/3T3 using 68214-1-Ig (same clone as 68214-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68214-1-PBS targets CCT2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rabbit, Rat samples.

Tested Reactivity Human, Mouse, Pig, Rabbit, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCT2 fusion protein Ag19264 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 2 (beta)
Calculated molecular weight 535 aa, 57 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC113516
Gene symbol CCT2
Gene ID (NCBI) 10576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with the molecular mass of 53 and 57 kDa. (PMID: 35699934)