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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CCT3 Monoclonal antibody

CCT3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5F5A6

Cat no : CL488-60264

Synonyms

TCP 1 gamma, Chaperonin containing T-complex polypeptide 1 subunit 3, CCT-gamma, CCTgamma, CCTG

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated CCT3 Monoclonal anti (CL488-60264)

    IF Staining of HepG2 using CL488-60264

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CCT3 antibody (CL488-60264, Clone: 5F5A6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated CCT3 Monoclonal anti (CL488-60264)

    FC experiment of HepG2 using CL488-60264

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CCT3 (CL488-60264, Clone:5F5A6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60264 targets CCT3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CCT3 fusion protein Ag0843 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 3 (gamma)
Calculated molecular weight 60 kDa
Observed molecular weight 56-60 kDa
GenBank accession numberBC006501
Gene symbol CCT3
Gene ID (NCBI) 7203
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CCT3 antibody CL488-60264Download protocol
FC protocol for CL Plus 488 CCT3 antibody CL488-60264Download protocol
Standard Protocols
Click here to view our Standard Protocols