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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT4 Monoclonal antibody, PBS Only

CCT4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rabbit, Pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C6A6

Cat no : 67455-1-PBS

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCT4 Monoclonal antibody (67455-1-Ig)

    WB analysis using 67455-1-Ig (same clone as 67455-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67455-1-Ig (CCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67455-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCT4 Monoclonal antibody (67455-1-Ig)

    IF Staining of U2OS using 67455-1-Ig (same clone as 67455-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT4 antibody (67455-1-Ig, Clone: 3C6A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67455-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67455-1-PBS targets CCT4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCT4 fusion protein Ag17084 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 4 (delta)
Calculated molecular weight 539 aa, 58 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC106934
Gene symbol CCT4
Gene ID (NCBI) 10575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.