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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT4 Monoclonal antibody

CCT4 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rabbit, Pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C6A6

Cat no : 67455-1-Ig

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCT4 Monoclonal antibody (67455-1-Ig)

    WB analysis using 67455-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67455-1-Ig (CCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCT4 Monoclonal antibody (67455-1-Ig)

    IF Staining of U2OS using 67455-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT4 antibody (67455-1-Ig, Clone: 3C6A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, mouse brain tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67455-1-Ig targets CCT4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCT4 fusion protein Ag17084 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 4 (delta)
Calculated molecular weight 539 aa, 58 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC106934
Gene symbol CCT4
Gene ID (NCBI) 10575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.

Protocols

Product Specific Protocols
WB protocol for CCT4 antibody 67455-1-IgDownload protocol
IF protocol for CCT4 antibody 67455-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols