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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CCT4 Monoclonal antibody

CCT4 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C6A6

Cat no : CL488-67455

Synonyms

Stimulator of TAR RNA-binding, SRB, Chaperonin containing T-complex polypeptide 1 subunit 4, CCT-delta, CCTD

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated CCT4 Monoclonal anti (CL488-67455)

    IF Staining of U2OS using CL488-67455

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 CCT4 antibody (CL488-67455, Clone: 3C6A6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated CCT4 Monoclonal anti (CL488-67455)

    FC experiment of A549 using CL488-67455

    1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cct4 Monoclonal Antibody (CL488-67455, Clone:3C6A6)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67455 targets CCT4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig, rabbit samples.

Tested Reactivity human, mouse, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCT4 fusion protein Ag17084 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 4 (delta)
Calculated molecular weight 539 aa, 58 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC106934
Gene symbol CCT4
Gene ID (NCBI) 10575
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CCT4 antibody CL488-67455Download protocol
FC protocol for CL Plus 488 CCT4 antibody CL488-67455Download protocol
Standard Protocols
Click here to view our Standard Protocols