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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT5 Recombinant antibody, PBS Only (Capture)

CCT5 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241510B3

Cat no : 84310-2-PBS

Synonyms

KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CCT5 Recombinant antibody (84310-2-RR)

    WB analysis using 84310-2-RR (same clone as 84310-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84310-2-RR (CCT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84310-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01209-1

    Sandwich ELISA standard curve of MP01209-1

    Sandwich ELISA standard curve of MP01209-1, Human CCT5 Recombinant Matched Antibody Pair - PBS only. 84310-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2146. 84310-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL

  • Sample test of MP01209-1

    Sample test of MP01209-1

    The mean CCT5 concentration was determined to be 28.01 ng/mL in U2OS cell extract based on a 3.1 mg/mL extract load and 35.60 ng/mL in HEK-293 cell extract based on a 2.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP01209-1

    Cytometric bead array standard curve of MP01209-1

    Cytometric bead array standard curve of MP01209-1, CCT5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84310-2-PBS. Detection antibody: 84310-1-PBS. Standard: Ag2146. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 84310-2-RR

    Affinity and Kinetic Characterization of 84310-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84310-2-RR against Human CCT5 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84310-2-PBS targets CCT5 as part of a matched antibody pair:

MP01209-1: 84310-2-PBS capture and 84310-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCT5 fusion protein Ag2146 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Calculated molecular weight 541 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC035499
Gene symbol CCT5
Gene ID (NCBI) 22948
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.