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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCT5 Recombinant antibody

CCT5 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241510B3

Cat no : 84310-2-RR

Synonyms

KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CCT5 Recombinant antibody (84310-2-RR)

    WB analysis using 84310-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84310-2-RR (CCT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84310-2-RR

    Affinity and Kinetic Characterization of 84310-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84310-2-RR against Human CCT5 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inA431 cells, HEK-293T cells, HeLa cells, Jurkat cells, A549 cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84310-2-RR is CCT5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCT5 fusion protein Ag2146 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Calculated molecular weight 541 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC035499
Gene symbol CCT5
Gene ID (NCBI) 22948
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

Protocols

Product Specific Protocols
WB protocol for CCT5 antibody 84310-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols