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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CCT8 Monoclonal antibody

CCT8 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A7C3

Cat no : CL488-67539

Synonyms

D21S246, Chaperonin containing T-complex polypeptide 1 subunit 8, CCT-theta, CCTQ, CCT theta

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated CCT8 Monoclonal anti (CL488-67539)

    IF Staining of MCF-7 using CL488-67539

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 CCT8 antibody (CL488-67539, Clone: 1A7C3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated CCT8 Monoclonal anti (CL488-67539)

    FC experiment of MCF-7 using CL488-67539

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CCT8 (CL488-67539, Clone:1A7C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67539 targets CCT8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCT8 fusion protein Ag30082 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 8 (theta)
Calculated molecular weight 548 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC012584
Gene symbol CCT8
Gene ID (NCBI) 10694
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CCT8 antibody CL488-67539Download protocol
FC protocol for CL Plus 488 CCT8 antibody CL488-67539Download protocol
Standard Protocols
Click here to view our Standard Protocols