APC Anti-Mouse CD107a / LAMP1 (1D4B)
CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse and More (1)
Applications
IF/ICC, FC (Intra)
Conjugate
APC Fluorescent Dye
CloneNo.
1D4B
Cat no : APC-65050
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
| Positive FC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
APC-65050 targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Plasma membrane fraction of mouse embryo NIH/3T3 cell line 相同性解析による交差性が予測される生物種 |
| Full Name | lysosomal-associated membrane protein 1 |
| GenBank accession number | BC006785 |
| Gene symbol | CD107a |
| Gene ID (NCBI) | 16783 |
| RRID | AB_2882955 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and a stabilizer. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APC CD107a / LAMP1 antibody APC-65050 | Download protocol |
| FC protocol for APC CD107a / LAMP1 antibody APC-65050 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharmacol Res RHBDF1 deficiency suppresses melanoma glycolysis and enhances efficacy of immunotherapy by facilitating glucose-6-phosphate isomerase degradation via TRIM32 |
