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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD107b / LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

Unconjugated

CloneNo.

H4B4

Cat no : 65053-1-Ig

Synonyms

CD107b, LAMP2, LAMP-2, LAMP 2, H4B4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using Anti-Human CD107b / LAMP2 (H4B4) (65053-1-Ig)

    IF Staining of U-251 using 65053-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CD107b / LAMP2 antibody (65053-1-Ig, Clone: H4B4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Anti-Human CD107b / LAMP2 (H4B4) (65053-1-Ig)

    IF Staining of HeLa using 65053-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65053-1-Ig (CD107b antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Anti-Human CD107b / LAMP2 (H4B4) (65053-1-Ig)

    FC experiment of HeLa using 65053-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107b / LAMP2 (65053-1-Ig, Clone:H4B4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Tested Applications

Positive IF/ICC detected inHeLa cells, U-251 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:100-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65053-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
RRIDAB_2881469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for CD107b / LAMP2 antibody 65053-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols