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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Human CD107b / LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

IF/ICC, FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

H4B4

Cat no : FITC-65053

Synonyms

LAMP2, CD107b, H4B4, LAMP 2, CD107 antigen-like family member B, LAMP-2, LAMPB, LGP110, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FITC Plus Anti-Human CD107b / LAMP2 (H4B4) (FITC-65053)

    IF Staining of HeLa using FITC-65053

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65053 (CD107b antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD107b / LAMP2 (H4B4) (FITC-65053)

    FC experiment of human PBMCs using FITC-65053

    1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107b (FITC-65053, Clone: H4B4). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD107b / LAMP2 (H4B4) (FITC-65053)

    FC experiment of human PBMCs using FITC-65053

    1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107b (FITC-65053, Clone: H4B4). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65053 targets CD107b / LAMP2 in IF/ICC, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
RRIDAB_2883743
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for FITC Plus CD107b / LAMP2 antibody FITC-65053Download protocol
Standard Protocols
Click here to view our Standard Protocols