APC Anti-Mouse CD107b / LAMP2 (ABL-93)
CD107b / LAMP2 Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Rat / IgG2a
Reactivity
Mouse
Applications
IF/ICC, FC (Intra)
Conjugate
APC Fluorescent Dye
CloneNo.
ABL-93
Cat no : APC-65052
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | NIH/3T3 cells |
Positive FC (Intra) detected in | NIH/3T3 cells |
Positive FC detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
APC-65052 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Host / Isotype | Rat / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Glycoproteins purified from BALB/c mouse embryo 3T3 cell line 相同性解析による交差性が予測される生物種 |
Full Name | lysosomal-associated membrane protein 2 |
GenBank accession number | BC138718 |
Gene symbol | CD107b |
Gene ID (NCBI) | 16784 |
RRID | AB_2882956 |
Conjugate | APC Fluorescent Dye |
Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and a stabilizer. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).
Protocols
Product Specific Protocols | |
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IF protocol for APC CD107b / LAMP2 antibody APC-65052 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |