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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD116 (4H1)

CD116 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

4H1

Cat no : PE-65160

Synonyms

CD116, CDw116, CSF2R, CSF2RA, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM CSF R alpha, GMCSFR, GMCSFR alpha, GMR, GMR alpha

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using PE Anti-Human CD116 (4H1) (PE-65160)

    FC experiment of human PBMCs using PE-65160

    1X10^6 human PBMCs were surface stained with 10 ul PE Anti-Human CD116 (PE-65160, Clone:4H1) (red), or PE Mouse IgG1 Isotype Control (blue), or unstained (black). Cells were not fixed. Monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65160 targets CD116 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen CD116-transfected COS cells 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Calculated molecular weight 46 kDa
GenBank accession numberBC002635
Gene symbol CD116
Gene ID (NCBI) 1438
RRIDAB_2883938
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for PE CD116 antibody PE-65160Download protocol
Standard Protocols
Click here to view our Standard Protocols