PE Anti-Mouse CD117/c-Kit (2B8)
CD117/c-Kit Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
mouse
Applications
FC
Conjugate
PE Fluorescent Dye
CloneNo.
2B8
Cat no : PE-65054
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | mouse bone marrow cells |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
PE-65054 targets CD117/c-Kit in FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rat / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Mouse IL-3-dependent bone marrow mast cells 相同性解析による交差性が予測される生物種 |
Full Name | kit oncogene |
GenBank accession number | BC052457 |
Gene symbol | CD117 |
Gene ID (NCBI) | 16590 |
RRID | AB_2883851 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).
Protocols
Product Specific Protocols | |
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FC protocol for PE CD117/c-Kit antibody PE-65054 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |