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Validation Data Gallery

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  • Western Blot (WB) analysis of TF-1 cells using CD11B/Integrin Alpha M Monoclonal antibody (66519-1-Ig)

    WB analysis of TF-1 using 66519-1-Ig (same clone as 66519-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human bone marrow tissue using CD11B/Integrin Alpha M Monoclonal antibody (66519-1-Ig)

    WB analysis of human bone marrow using 66519-1-Ig (same clone as 66519-1-PBS)

    human bone marrow tissue were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11B/Integrin Alpha M Monoclonal antibody (66519-1-Ig)

    IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11B/Integrin Alpha M Monoclonal antibody (66519-1-Ig)

    IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11B/Integrin Alpha M Monoclonal antibody (66519-1-Ig)

    IF Staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11B/Integrin Alpha M Monoclonal antibody (66519-1-Ig)

    IF Staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD11B/Integrin Alpha M antibody (66519-1-Ig, Clone: 1C7C2 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

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CD11B/Integrin Alpha M Monoclonal antibody, PBS Only

CD11B/Integrin Alpha M Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66519-1-PBS
Clone No.1C7C2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD11b, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, CD11 antigen-like family member B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66519-1-PBS targets CD11B/Integrin Alpha M in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 93 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_000632
Gene Symbol CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
RRIDAB_2881882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11215
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).