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CD11B/Integrin Alpha M Monoclonal antibody

CD11B/Integrin Alpha M Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66519-1-Ig
Clone No.1C7C2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

CD11b, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, CD11 antigen-like family member B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inTF-1 cells, human bone marrow tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 93 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_000632
Gene Symbol CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
RRIDAB_2881882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11215
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
WB protocol for CD11B/Integrin Alpha M antibody 66519-1-IgDownload protocol
IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-IgDownload protocol
IF protocol for CD11B/Integrin Alpha M antibody 66519-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

J Exp Med

TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.

Authors - Dehong Yan
humanIF

Cell Rep

Inflammatory dendritic cells restrain CD11b+CD4+ CTLs via CD200R in human NSCLC

Authors - Mingjie Lin
FC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
IF

Apoptosis

ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments

Authors - Qian Yan
WB

J Neuroinflammation

C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation.

Authors - Liu Ye
humanWB

Int Immunopharmacol

Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1

Authors - Shoufang Tong
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