CD11B/Integrin Alpha M Monoclonal antibody
CD11B/Integrin Alpha M Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1C7C2
Cat no : 66519-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, TF-1 cells, human bone marrow tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
| Positive FC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 19 publications below |
| FC | See 1 publications below |
Product Information
66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Calculated molecular weight | 93 kDa |
| Observed molecular weight | 160 kDa |
| GenBank accession number | NM_000632 |
| Gene symbol | ITGAM |
| Gene ID (NCBI) | 3684 |
| RRID | AB_2881882 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| IF protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| FC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. | ||
Bioact Mater Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect. | ||
Apoptosis ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments | ||
J Neuroinflammation C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Santhosh (Verified Customer) (04-15-2019) | Human FFPE tonsil tissue, HIER pH6
 |
