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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a Recomb (Biotin-65563)

    FC experiment of human peripheral blood leukocytes using Biotin-65563

    1x10^6 human peripheral blood leukocytes were unstained or surface stained with 0.25 ug Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a RecAb (Biotin-65563, Clone: TS2/4) and CoraLite®488-conjugated streptavidin. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a Recomb (Biotin-65563)

    FC experiment of human peripheral blood leukocytes using Biotin-65563

    1x10^6 human peripheral blood leukocytes were unstained (blue) or surface stained with 5 ul Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a RecAb (Biotin-65563, Clone: TS2/4) and CoraLite®488-conjugated streptavidin (red). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody

CD11a Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-65563
Clone No.TS2/4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65563 targets CD11a in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen HLA-DR CTL line 相同性解析による交差性が予測される生物種
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated molecular weight 129 kDa
GenBank accession numberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20701
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
FC protocol for Biotin CD11a antibody Biotin-65563Download protocol
Standard Protocols
Click here to view our Standard Protocols