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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody

CD11b Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

ICRF44

Cat no : 65582-1-MR

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant (65582-1-MR)

    FC experiment of human peripheral blood leukocyte using 65582-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11b Mouse IgG2a Recombinant Antibody (65582-1-MR, Clone: ICRF44) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant (65582-1-MR)

    FC experiment of human peripheral blood leukocyte using 65582-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11b Mouse IgG2a Recombinant Antibody (65582-1-MR, Clone: ICRF44)(red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant (65582-1-MR)

    FC experiment of human peripheral blood leukocyte using 65582-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11b Mouse IgG2a Recombinant Antibody (65582-1-MR, Clone: ICRF44)(left) or 0.25 ug Anti-Human CD11b (65116-1-Ig, Clone: ICRF44) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65582-1-MR targets CD11b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 1152 aa, 127 kDa
GenBank accession numberBC096346
Gene symbol ITGAM
Gene ID (NCBI) 3684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
FC protocol for CD11b antibody 65582-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols