CoraLite® Plus 488 Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody
CD11b Recombinant Antibody for FC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
ICRF44
Cat no : CL488-65582
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-65582 targets CD11b in FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Recombinant |
Type | Antibody |
Immunogen | Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種 |
Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
Calculated molecular weight | 1152 aa, 127 kDa |
GenBank accession number | BC096346 |
Gene symbol | CD11b |
Gene ID (NCBI) | 3684 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD11b antibody CL488-65582 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |