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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD11b (ICRF44)

CD11b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

ICRF44

Cat no : CL647-65116

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood granulocytes using CoraLite® Plus 647 Anti-Human CD11b (ICRF44) (CL647-65116)

    FC experiment of human peripheral blood granulocytes using CL647-65116

    1X10^6 human peripheral blood granulocytes were surface stained with 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD11b (CL647-65116, Clone:ICRF44) (red) or isotype control antibody (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of THP-1 cells using CoraLite® Plus 647 Anti-Human CD11b (ICRF44) (CL647-65116)

    FC experiment of THP-1 using CL647-65116

    1X10^6 unstimulated (dashed line) or PMA treated THP-1 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD11b (CL647-65116, Clone: ICRF44) (red), or isotype control (blue, solid line). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood granulocytes, PMA treated THP-1 cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65116 targets CD11b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 1152 aa, 127 kDa
GenBank accession numberBC096346
Gene symbol CD11b
Gene ID (NCBI) 3684
RRIDAB_2883692
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD11b antibody CL647-65116Download protocol
Standard Protocols
Click here to view our Standard Protocols