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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PerCP-Cyanine5.5 Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

M1/70

Cat no : CPY5-65055

Synonyms

M1/70, Ly 40, Itgam, integrin alpha M, CR3A

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse bone marrow cells using PerCP-Cyanine5.5 Anti-Mouse CD11b (M1/70) (CPY5-65055)

    FC experiment of mouse bone marrow cells using CPY5-65055

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug PerCP-Cyanine5.5 Anti-Mouse CD11b (M1/70) (CPY5-65055, Clone:M1/70) or unstained. Cells were then stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (CL647-65140, Clone: RB6-8C5). Cells were not fixed.

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Tested Applications

Positive FC detected inmouse bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65055 targets CD11b in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 相同性解析による交差性が予測される生物種
Full Name integrin alpha M
Calculated molecular weight127 kDa
GenBank accession numberBC156991
Gene symbol CD11b
Gene ID (NCBI) 16409
Conjugate PerCP-Cyanine5.5 Fluorescent Dye
Excitation/Emission maxima wavelengths482 nm / 690 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
FC protocol for PerCP-Cyanine5.5 CD11b antibody CPY5-65055Download protocol
Standard Protocols
Click here to view our Standard Protocols