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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD11c (3.9)

CD11c Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3.9

Cat no : CL647-65086

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood monocytes using CoraLite® Plus 647 Anti-Human CD11c (3.9) (CL647-65086)

    FC experiment of human peripheral blood monocytes using CL647-65086

    100 ul human peripheral blood were surface stained with 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD11c (CL647-65086, Clone:3.9) and then treated with red blood cell lysis buffer. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood monocytes using CoraLite® Plus 647 Anti-Human CD11c (3.9) (CL647-65086)

    FC experiment of human peripheral blood monocytes using CL647-65086

    100 ul human peripheral blood were surface stained with CoraLite® Plus 647-conjugated Mouse IgG1 isotype control and then treated with red blood cell lysis buffer. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood monocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65086 targets CD11c in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
GenBank accession numberBC038237
Gene symbol CD11c
Gene ID (NCBI) 3687
RRIDAB_2883672
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD11c antibody CL647-65086Download protocol
Standard Protocols
Click here to view our Standard Protocols