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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD122/IL2RB Monoclonal antibody, PBS Only

CD122/IL2RB Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12C7

Cat no : 60174-1-PBS

Synonyms

CD122/IL2RB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD122/IL2RB Monoclonal antibody (60174-1-Ig)

    WB analysis using 60174-1-Ig (same clone as 60174-1-PBS)

    Untreated and PNGase F-treated lysates of NK-92 cells were subjected to SDS PAGE followed by western blot with 60174-1-Ig (CD122/IL2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60174-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using CD122/IL2RB Monoclonal antibody (60174-1-Ig)

    WB analysis of Recombinant protein using 60174-1-Ig (same clone as 60174-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60174-1-Ig (IL2RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60174-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60174-1-PBS targets CD122/IL2RB in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD122/IL2RB fusion protein Ag3929 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, beta
Calculated molecular weight 551 aa, 61 kDa
Observed molecular weight75 kDa, 61 kDa
GenBank accession numberBC025691
Gene symbol IL2RB
Gene ID (NCBI) 3560
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.