Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
60174-1-PBS targets CD122/IL-2RB as part of a matched antibody pair:
MP50451-1: 60174-3-PBS capture and 60174-1-PBS detection (validated in Cytometric bead array)
MP50451-2: 60174-2-PBS capture and 60174-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD122/IL-2RB fusion protein Ag3929 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 2 receptor, beta |
| Calculated molecular weight | 551 aa, 61 kDa |
| Observed molecular weight | 75 kDa, 61 kDa |
| GenBank accession number | BC025691 |
| Gene Symbol | IL2RB |
| Gene ID (NCBI) | 3560 |
| ENSEMBL Gene ID | ENSG00000100385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14784 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.