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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD122/IL-2RB Monoclonal antibody

CD122/IL-2RB Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A12C7

Cat no : 60174-1-Ig

Synonyms

IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD122/IL2RB Monoclonal antibody (60174-1-Ig)

    WB analysis using 60174-1-Ig

    Untreated and PNGase F-treated lysates of NK-92 cells were subjected to SDS PAGE followed by western blot with 60174-1-Ig (CD122/IL2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Recombinant protein using CD122/IL2RB Monoclonal antibody (60174-1-Ig)

    WB analysis of Recombinant protein using 60174-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60174-1-Ig (IL2RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inNK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60174-1-Ig targets CD122/IL-2RB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD122/IL-2RB fusion protein Ag3929 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, beta
Calculated molecular weight 551 aa, 61 kDa
Observed molecular weight75 kDa, 61 kDa
GenBank accession numberBC025691
Gene symbol IL2RB
Gene ID (NCBI) 3560
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.

Protocols

Product Specific Protocols
WB protocol for CD122/IL-2RB antibody 60174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols