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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Human CD13 (WM15)

CD13 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

WM15

Cat no : APC-65186

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

Validation Data Gallery

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  • Flow cytometry (FC) experiment of whole blood using APC Anti-Human CD13 (WM15) (APC-65186)

    FC experiment of whole blood using APC-65186

    Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 5 ul APC Anti-Human CD13 (WM15) (APC-65186) or APC Mouse IgG1 Isotype Control. Cells were not fixed. Granulocytes were gated.

  • Flow cytometry (FC) experiment of whole blood using APC Anti-Human CD13 (WM15) (APC-65186)

    FC experiment of whole blood using APC-65186

    Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 5 ul APC Anti-Human CD13 (WM15) (APC-65186) or APC Mouse IgG1 Isotype Control. Cells were not fixed. Granulocytes were gated.

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Tested Applications

Positive FC detected inhuman peripheral blood granulocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65186 targets CD13 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human AML cells 相同性解析による交差性が予測される生物種
Full Name alanyl (membrane) aminopeptidase
Calculated molecular weight 110 kDa
GenBank accession numberBC058928
Gene symbol CD13
Gene ID (NCBI) 290
RRIDAB_2883046
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

Protocols

Product Specific Protocols
FC protocol for APC CD13 antibody APC-65186Download protocol
Standard Protocols
Click here to view our Standard Protocols