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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CoraLite® Plus 488-conjugated CD133 Polyclonal ant (CL488-18470)

    IF Staining of mouse kidney using CL488-18470

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite® Plus 488 CD133 antibody (CL488-18470) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CD133 Polyclonal antibody

CD133 Polyclonal Antibody for IF-P
Cat No. CL488-18470

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Synonyms

PROM1, MSTP061, MCDR2, CORD12, Antigen AC133

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18470 targets CD133 in IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD133 fusion protein Ag13328 相同性解析による交差性が予測される生物種
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight
GenBank accession numberBC012089
Gene Symbol CD133
Gene ID (NCBI) 8842
RRIDAB_2919158
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43490
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD133 antibody CL488-18470Download protocol
Standard Protocols
Click here to view our Standard Protocols