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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using CD14 Recombinant antibody (83297-8-RR)

    WB analysis of RAW 264.7 using 83297-8-RR (same clone as 83297-8-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83297-8-RR (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83297-8-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD14 Recombinant antibody (83297-8-RR)

    IF Staining of RAW 264.7 using 83297-8-RR (same clone as 83297-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-8-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83297-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83297-8-RR

    Affinity and Kinetic Characterization of 83297-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83297-8-RR against Mouse CD14 were performed. The affinity constant is 0.219 nM.

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Mouse CD14 Recombinant antibody, PBS Only

CD14 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83297-8-PBS
Clone No.240225C2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CD14 antigen, 240225C2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83297-8-PBS targets CD14 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD14 antigen
Calculated molecular weight39 kDa
Observed molecular weight45-55 kDa
GenBank accession numberNM_009841.4
Gene Symbol Cd14
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10810
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.