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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD14 Recombinant antibody

CD14 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240225C2

Cat no : 83297-8-RR

Synonyms

CD14 antigen, 240225C2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using CD14 Recombinant antibody (83297-8-RR)

    WB analysis of RAW 264.7 using 83297-8-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83297-8-RR (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD14 Recombinant antibody (83297-8-RR)

    IF Staining of RAW 264.7 using 83297-8-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-8-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83297-8-RR

    Affinity and Kinetic Characterization of 83297-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83297-8-RR against Mouse CD14 were performed. The affinity constant is 0.219 nM.

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Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83297-8-RR is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD14 antigen
Calculated molecular weight39 kDa
Observed molecular weight45-55 kDa
GenBank accession numberNM_009841.4
Gene symbol Cd14
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 83297-8-RRDownload protocol
IF protocol for CD14 antibody 83297-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols