Anti-Human CD14 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CD14 Rabbit Recombinant Antibody, PBS Only
Brand: Proteintech
SKU: 98040-1-PBS

CD14 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Conjugate

Unconjugated

CloneNo.

230332D7

Review Product

Cat no : 98040-1-PBS

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D7

Validation Data Gallery

Filter:

AllFCAffinity

FC experiment of human PBMCs using 98040-1-RR (same clone as 98040-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98040-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98040-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98040-1-RR (same clone as 98040-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7)(red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98040-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7)(red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98040-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98040-1-RR
Biolayer interferometry (BLl) kinetic assays of 98040-1-RR against Human CD14 were performed. The affinity constant is 0.703 nM.

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Cat No. 98040-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98040-1-PBS is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Fusion Protein 相同性解析による交差性が予測される生物種
Full Name	CD14 molecule
Calculated molecular weight	375 aa, 40 kDa
GenBank accession number	BC010507
Gene symbol	CD14
Gene ID (NCBI)	929
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

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