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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD14 Rabbit Recombinant Antibody

CD14 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230332D7

Cat no : 98040-1-RR

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR)

    FC experiment of human PBMCs using 98040-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR)

    FC experiment of human PBMCs using 98040-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7)(red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98040-1-RR

    Affinity and Kinetic Characterization of 98040-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98040-1-RR against Human CD14 were performed. The affinity constant is 0.703 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98040-1-RR is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for CD14 antibody 98040-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols