Atlantic Blue™ Anti-Human CD14 (UCHM-1)
CD14 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
FC
Conjugate
Atlantic Blue™ Fluorescent Dye
CloneNo.
UCHM-1
Cat no : AB-65056
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
AB-65056 targets CD14 in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Thymocytes and peripheral blood lymphocytes from a remission AML patient 相同性解析による交差性が予測される生物種 |
Full Name | CD14 molecule |
Calculated molecular weight | 375 aa, 40 kDa |
GenBank accession number | BC010507 |
Gene symbol | CD14 |
Gene ID (NCBI) | 929 |
Conjugate | Atlantic Blue™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 404 nm / 458 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
Protocols
Product Specific Protocols | |
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FC protocol for Atlantic Blue™ CD14 antibody AB-65056 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |