• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a Rec (Biotin-65616)

    FC experiment of human PBMCs using Biotin-65616

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC) and CoraLite®488-conjugated streptavidin, or unstained cells. Cells were co-stained with APC Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a Rec (Biotin-65616)

    FC experiment of human PBMCs using Biotin-65616

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC)(red) and CoraLite®488-conjugated streptavidin, or unstained cells(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

Biotin Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody

CD14 Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-65616
Clone No.26IC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65616 targets CD14 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
GenBank accession numberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for Biotin CD14 antibody Biotin-65616Download protocol
Standard Protocols
Click here to view our Standard Protocols