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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD146 (P1H12)

CD146 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rabbit, Canine, Mouse, Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

P1H12

Cat no : CL647-65181

Synonyms

CD146, MCAM, MUC18

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A375 cells using CoraLite® Plus 647 Anti-Human CD146 (P1H12) (CL647-65181)

    FC experiment of A375 using CL647-65181

    1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD146 (CL647-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

  • Flow cytometry (FC) experiment of A375 cells using CoraLite® Plus 647 Anti-Human CD146 (P1H12) (CL647-65181)

    FC experiment of A375 using CL647-65181

    1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD146 (CL647-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

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Tested Applications

Positive FC detected inA375 cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65181 targets CD146 in FC applications and shows reactivity with Rabbit, Canine, Mouse, Human samples.

Tested Reactivity Rabbit, Canine, Mouse, Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen cultured human umbilical cells 相同性解析による交差性が予測される生物種
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
GenBank accession numberBC056418
Gene symbol CD146
Gene ID (NCBI) 4162
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD146 antibody CL647-65181Download protocol
Standard Protocols
Click here to view our Standard Protocols