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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD147 Recombinant antibody, PBS Only

CD147 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230046A9

Cat no : 82909-7-PBS

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD147 Recombinant antibody (82909-7-RR)

    WB analysis using 82909-7-RR (same clone as 82909-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82909-7-RR (CD147 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD147 Recombinant antibody (82909-7-RR)

    IF Staining of HeLa using 82909-7-RR (same clone as 82909-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

  • Affinity experiments of 82909-7-RR

    Affinity experiments of 82909-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82909-7-RR against Human CD147 were performed. The affinity constant is 21.7 nM.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 82909-7-PBS is CD147 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name basigin (Ok blood group)
Calculated molecular weight 385 aa, 42 kDa
GenBank accession numberBC009040
Gene symbol BSG
Gene ID (NCBI) 682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.