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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD147 Recombinant antibody

CD147 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230046A9

Cat no : 82909-7-RR

Synonyms

EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD147 Recombinant antibody (82909-7-RR)

    WB analysis using 82909-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82909-7-RR (CD147 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD147 Recombinant antibody (82909-7-RR)

    IF Staining of HeLa using 82909-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD147 Recombinant antibody (82909-7-RR)

    IF Staining of HeLa using 82909-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82909-7-RR

    Affinity and Kinetic Characterization of 82909-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82909-7-RR against Human CD147 were performed. The affinity constant is 21.7 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82909-7-RR is CD147 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name basigin (Ok blood group)
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight50-60 kDa
GenBank accession numberBC009040
Gene symbol CD147
Gene ID (NCBI) 682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).

Protocols

Product Specific Protocols
WB protocol for CD147 antibody 82909-7-RRDownload protocol
IF protocol for CD147 antibody 82909-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols