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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD147 Monoclonal antibody (CL594-66443)

    IF Staining of human tonsillitis using CL594-66443

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD147 antibody (CL594-66443, Clone: 1G12B5 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD147 Monoclonal antibody (CL594-66443)

    IF Staining of human tonsillitis using CL594-66443

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD147 antibody (CL594-66443, Clone: 1G12B5 ) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CD147 Monoclonal antibody

CD147 Monoclonal Antibody for IF-P
Cat No. CL594-66443
Clone No.1G12B5

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF-P

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66443 targets CD147 in IF-P applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD147 fusion protein Ag2619 相同性解析による交差性が予測される生物種
Full Name basigin (Ok blood group)
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC009040
Gene Symbol CD147
Gene ID (NCBI) 682
RRIDAB_2883583
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35613
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BSG, also named as 5F7, Basigin, EMMPRIN, TCSF and CD147, plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA, inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix, plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.

Protocols

Product Specific Protocols
IF protocol for CL594 CD147 antibody CL594-66443Download protocol
FC protocol for CL594 CD147 antibody CL594-66443Download protocol
Standard Protocols
Click here to view our Standard Protocols